Frontiers

Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach

We have recently introduced a novel approach to time-resolved cryo-electron microscopy (cryo-EM) that affords microsecond time resolution. It involves melting a cryo sample with a laser beam to allow ...
Europe PMC

Microsecond MD simulations of human CYP2D6 wild-type and five allelic variants reveal mechanistic insights on the function.

Characterization of cytochrome P450 2D6 (CYP2D6) and the impact of the major identified allelic variants on the activity of one of the most dominating drug-metabolising enzymes is essential to ...
PNAS

Using microsecond single-molecule FRET to determine the assembly pathways of T4 ssDNA binding protein onto model DNA replication forks

A microsecond-resolved single-molecule FRET method was used to monitor the binding and unbinding of the ssDNA binding protein (gene product 32) of the T4 bacteriophage replication complex to ...
PNAS

Microsecond and millisecond dynamics in the photosynthetic protein LHCSR1 observed by single-molecule correlation spectroscopy

Protein flexibility is essential for the robustness of biological systems, yet the dynamics underlying this flexibility are difficult to observe, because they are small, fast, and stochastic.
C&EN

Temperature-Jump Fluorescence Provides Evidence for Fully Reversible Microsecond Dynamics in a Thermophilic Alcohol Dehydrogenase

∥ Department of Chemistry, University of Washington, Seattle, Washington 98195, United States Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF ...