Pyrosequencing technique is based on sequencing-by-synthesis principle. The method is based on the sequencing of single stranded DNA and now-a-days sequencing of double stranded DNA is also possible.

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New Delhi: Each DNA strand is made of 4 chemical units called nucleotide bases which compromise the GENETIC ALPHABET. These bases are Adenine(A), Guanine (G), Cytosine(C), and Thymine(T). The sequence ...

Genotypic testing is recommended as the preferred resistance testing method to guide therapy in antiretroviral-naive and antiretroviral-experienced persons. A major limitation of clinically available ...