The expression of foreign proteins in the fission yeast, Schizosaccharomyces pombe, is achieved by introducing an expression vector along with a transducing vector containing an autonomously ...

Because the pCMV-Script vector contains the neo gene encoding G418 resistance, DNA transfection of the vector itself into cancer cells resulted in a number of colonies formed in the presence of G418.

Here we present a modified vector pCMV-3Taq-LIC for a rapid, simple, and relatively cheap method to build expression constructs. After being digested by Nt.BspQI and EcoRV, a lineal vector with ...